Viruses : Collection and Processing of Specimens for Viral Diagnosis

Viruses : Collection and Processing of Specimens for Viral Diagnosis

a Vesicles are cleaned with 70% alcohol followed by sterile saline. Viruses are obtained by unroofing
a vesicle with a needle or a scalpel blade. The fluid is collected with a swab or with a tuberculin syringe with a 26 to 27-gauge needle

- The fluid obtained from fresh vesicles may contain enough viruses for culture
- Direct smears are prepared by scraping cells form the base of the lesions
- The cells are smeared on a slide, fixed and stained with Giemsa or Wright stains to identify multi cellularar cells with inclusion bodies, or procedures of specific antibodies conjugated to fluoresce or to immunoperoxidase methods may also be used.

Collection and processing of specimens for viral Diagnosis

- Select a freshly erupted vesicle, preferably containing clear fluid

- Carefully puncture the vesicle and collect the fluid on a cotton wool swab

- Rub the base of the lesion with the same swab

- Break off the head of swab into viral transport medium (VTM) and transport to laboratory as soon as possible

- The date of request, the date and time of collection and finally brief clinical information and provisional diagnosis should be stated with the sample.

- Scrapings may also be taken from the base or side of the lesion with a small curetting spoon or fine scalpel;

- The harvested cells can then be smeared on slides for immuno fluorescence (IF) examination and the curette is agitated in virus transport medium to provide samples for culture-amplified EIA.

- The epithelial cells most likely to contain inclusions and antigens are those taken from the advancing edge of the lesion.

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Viruses : Collection and Processing of Specimens for Viral Diagnosis 2018-12-26T01:05:00-08:00 Rating: 4.5 Diposkan Oleh: David Maharoni